primary human satellite cells  (Innoprot Inc)

Journal: Cell Proliferation

Article Title: HIPK2 is involved in cell proliferation and its suppression promotes growth arrest independently of DNA damage

doi: 10.1111/j.1365-2184.2009.00601.x

Figure Lengend Snippet: HIPK2 expression in skeletal muscle and haemopoietic differentiation. Western blot analyses of indicated proteins were performed on total cell extracts from proliferating (Prol) and differentiated cells of different lines: (a) mouse primary satellite cells; (b) human primary satellite cells; (c) human HL60 leukaemia cells; (d) mouse 32D myeloid progenitor cells. ‘% TD cells’ indicates percentage of terminally differentiated cells. (e) 32D cells were differentiated into granulocytes by incubation in the presence of G‐CSF as in (d) and total cell extracts were obtained by total population or after density gradient purification of live cells. Relative percentage of viability is indicated below. (f) Human primary satellite cells were cultured in growth medium or differentiation medium in the presence or absence of MG132; p53 expression was tested as positive control of MG132 activity. (g, h) Human primary satellite cells were transduced with HIPK2‐specific siRNA or with the relative control (UNC) and cultured in growth medium or differentiation medium as in (f). Real‐time RT‐PCR (g) and Western blot (h) analyses were performed on the indicated genes and proteins. Tubulin and actin were used as loading controls.

Article Snippet: Primary human satellite cells were purchased from Cambrex Bio Science Walkersville Inc. (Walkersville, MD, USA) and were cultured and differentiated following manufacturer's instructions to customers.

Techniques: Expressing, Western Blot, Incubation, Purification, Cell Culture, Positive Control, Activity Assay, Transduction, Control, Quantitative RT-PCR